Making culture on the Malt Extract and Agar (MEA) plates¶
I have this utopia to going back to forest collect mushrooms and making object, matter, medium with it. Was my first test and lot of contaminations was follow making beautiful colors.
In August, happy moment so-much gratitude to nature: paper mushrooms
References¶
- Annah Sangosanya, Fabricademy 2022
- Making Agar Plates and sterilization, Week9
- Sterilization for the material
Recipe¶
| Material & ingredients | Details |
|---|---|
| Ingredient for Malt Extract Agar | Distilled Water, Extract of Malt Powder, Agar Agar |
| Specific material for preparation | Balance, Spoon, Bowl, Hand mixer |
| Specific material for sterilisation | Jar with perforated cover, Petri dishes in glass, Aluminium paper, Autoclave |
| Specific material for inoculation | Camping gaz with lighter, Ethanol 70°, Absorbant paper, Masks, Good knife or scalpel, Petri dishes sterilized |
Recipe for 5 petri dishes (MEA).
Mix 5g Aguar and 5g of Malt Extract.
Boiled 250ml Distilled water.
Add the hot water in the Agar and Malt.
Mix it.
Put the preparation in the jar.
Close it with cover perforated and add aluminium paper on the top.
Add in auto cooking pot for sterilization of the preparation.
When it’s starting to be pshhhhhh, ower the heat.
Sterilise mixture for 20 minutes.
Stop and leave it during time to be ambient temperature.
Add the mixture MEA sterilized inside the petri dishes [already sterilized](https://class.textile-academy.org/2023/amandine-fery/Project-Development/Project-Workflow/02∙Treatment/#material)( in the little part)
Wear mask and gloves
Clean your mushroom and open it with the cutter.
Take the white part and add it on the petri dishes sterilized.
Close the petri dishes with the autoclave tape.
Add the date and the name of your mushroom you put inside.
Add them in the yogurt maker with towel on it.
Petri Dishies 1¶
| Fungal species | Feedstock | Sterilisation method | Innoculation and packing method | Growth condition and time | Drying method, Post traitment |
|---|---|---|---|---|---|
| Trametes Versicolor | MEA | 20min autoclave cooker | Innoculated into a petri dishes, camping gaz | Yogurt maker with towel on top, 26-28°, 25 days, in the dark | / |
Petri Dishies 2¶
| Fungal species | Feedstock | Sterilisation method | Innoculation and packing method | Growth condition and time | Drying method, Post traitment |
|---|---|---|---|---|---|
| Stereum Hirsutum | MEA | 20min autoclave cooker | Innoculated into a petri dishes, camping gaz | Yogurt maker with towel on top, 26-28°, 25 days, in the dark | / |
Petri Dishies 3¶
| Fungal species | Feedstock | Sterilisation method | Innoculation and packing method | Growth condition and time | Drying method, Post traitment |
|---|---|---|---|---|---|
| Grown bio kit Reishi | 150g of the spain grain + 50g of fabric cut | 1h autoclave cooker | Innoculated into a jar, camping gaz | Yogurt maker with towel on top, 26-28°, 30 days, in the dark | heat press 70° |
Last update:
2023-10-21