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Treatment, sterilization, inoculation

The sterilization is usefull for not adding others bacteria inside our work.

For the Scoby collect directly to the producer there are not particular sterilization. For more details to making it by ourself go in the week 6.

Sterilization for Mycelium

Petri dishes contaminated.


Materials and tools for sterilization

Material for sterilization Details
Consommable material Ethanol 70°, gaz, mask, absorbant paper
Material Spoon, balance, jar, good knife / scalpel
Sterilizator for the space Camping gaz, UVC lamp
Sterilizator for the material and substrate Autoclave cooker
Substrate coffee grain, spain grain, linen textile...

The principles things I taking care to sterilize are:

  • Material

  • Substrate

  • Space

  • Us

Material

19 Plastic petri dishes molted in the pressure cooker after 1 hour. Use glass...


For the sterilization of the little materials I use essentially the Ethanol; for the jar and petri dishes I use the autoclave cooker.


Substrate

For sterilized the substrate I use different process:


For the textile fabric sometimes I was washing it sometimes not. The natural fabrics are similar process to textile fabric.

Space

Cleaning space mean: cleaning the air and surface arround where we want to work.

I use different method for clean the space :

  • Using the camping gaz during the innoculation part and in the lab.

  • Using Ethanol for cleaning my work space

  • Using a UV lamp specially use in the lab, in Strate school where there lot of passage and others biomaterials arround

  • At home I only use ethanol or hydroalcoolic gel.

More info:

Laminar flow wood

Inspirations and researchs :

The laminar flow wood is using for cleaning air of others bacteries during manipulation of microbial. In the starter of the project I was aswell really considering to make it one for the lab. For the place, time and didn't know enough about mushrooms when I start I decided to didn't make it.

Camping Gaz

We have already camping gaz in the lab.

UVC Lamp

I bought one in the second hand shop. Is used for disinfection of air and certain surfaces to reduce the risk of infection by bacteria or viruses.

It is preferable to stay away from it during the time of its use by leaving the room. Use between 15 and 30min depending on the model.

I'm not really satisfy. I feel desoriented and sick when the lamp is working. I didn't recommand to buy and use it.


Inoculation

Inoculation is hading mycelium inside the substrate already sterilized.

Material & ingredients for inoculation Details
Consommable material Ethanol 70°, masks, gloves, marker
Material Spoon, balance, jar sterilized
Sterilizator for the space Camping gaz with lighter
Ingredients mycelium and substrate already sterilized
Wear a mask, gloves and be careful if you have a long hair to be atatched! 
Sterilize the place and material with the Ethanol. 
Turn on the camping gaz during the process
Add part of the mycelium inside the jar with substrate.
Close the jar
Identify
Store it

Last update: 2024-05-12